![]() coli, including the key factors for successful protein production, highlighting soluble protein production, and a comprehensive summary of the latest available and traditionally used gene fusion technologies. In this review, we provide an overview of current strategies to improve recombinant protein production in E. New fusion partners are constantly emerging and complementing the traditional solutions, as for instance, the Fh8 fusion tag that has been recently studied and ranked among the best solubility enhancer partners. coli, and for increasing peptide’s immunogenicity as well. Gene fusion technology has been widely used for the improvement of soluble protein production and/or purification in E. With the aid of genetic and protein engineering novel tailored-made strategies can be designed to suit user or process requirements. ![]() coli benefits of cost, ease of use and scale make it essential to design new approaches directed for improved recombinant protein production in this host cell. The Escherichia coli is still the dominant host for recombinant protein production but, as a bacterial cell, it also has its issues: the aggregation of foreign proteins into insoluble inclusion bodies is perhaps the main limiting factor of the E. ![]() Proteins are now widely produced in diverse microbial cell factories.
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June 2023
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